- Merck ZIC-HILIC 亲水作用色谱柱
- Merck ZIC-HILIC 亲水作用色谱柱
Merck ZIC-HILIC 亲水作用色谱柱
ZIC-HILIC默克 Merck ZIC-HILIC 亲水作用色谱柱
为什么使用HILIC?
尽管反相液相色谱(RPLC)是至今应用zui广的色谱分离技术,它能与各种常规的检测方法结合,解决多种分析应用问题,但对某些分析物,特别是极性和亲水性化合物却无法或很少保留。长期以来,人们采用正相液相色谱(NPLC),并使用不利环保的非水溶性流动相如己脘或环己脘来分析这些物质。
但是在这种实验条件下,很多极性和亲水化合物往往很难溶解,从而限制了NPLC的应用范围。从现在起有了另一种新的解决办法,即选用亲水作用液相色谱-HILIC。
什么是亲水作用色谱(HILIC):
亲水作用液色谱(Hydrophilic Interaction LiquidChromatography,HILIC) 是一种直接分离极性和亲水性化合物的色谱技术,为了便于理解,我们可以把它看作为是一种反相色谱溶剂做为流动相的正相色谱。
因此HILIC是:
●亲水性的固定相
●以水、有机溶剂和缓冲液作为流动相洗脱剂
常见的HILIC方法采用50-90%有机溶剂和一定比例的水,并且含有低浓度的缓冲盐。如乙腈/水/醋酸铵体系在HILIC方法中,洗脱顺序和反相色谱正好相反,即极性越大,保留时间越长。
ZIC-HILIC 和 ZIC-pHILIC两性离子固定相的官能团:
的应用:
订货信息:货号
描述固定相规格
1.50434.0001
ZIC®-HILIC (5µm) 14 x 1 mm PEEK保护柱(5件) fitting guard column
1.50435.0001
ZIC®-HILIC (5µm) 20 x 2.1 mm PEEK保护柱(1件) fitting guard column
1.50436.0001
ZIC®-HILIC (5µm) 20 x 2.1 mm PEEK保护柱套件(3件) fitting guard column
1.50439.0001
ZIC®-HILIC (3.5µm, 100Å) 20 x 2.1 mm PEEK HPLC column
1.50440.0001
ZIC®-HILIC (3.5µm, 100Å) 50 x 2.1 mm PEEK HPLC column
1.50441.0001
ZIC®-HILIC (3.5µm, 100Å) 100 x 2.1 mm PEEK HPLC column
1.50442.0001
ZIC®-HILIC (3.5µm, 100Å) 150 x 2.1 mm PEEK HPLC column
1.50444.0001
ZIC®-HILIC (3.5µm, 100Å) 150 x 4.6 mm PEEK HPLC column
1.50443.0001
ZIC®-HILIC (3.5µm, 100Å) 250 x 2.1 mm PEEK HPLC column
1.50445.0001
ZIC®-HILIC (3.5µm, 200Å) 50 x 2.1 mm PEEK HPLC column
1.50446.0001
ZIC®-HILIC (3.5µm, 200Å) 50 x 4.6 mm PEEK HPLC column
1.50447.0001
ZIC®-HILIC (3.5µm, 200Å) 100 x 2.1 mm PEEK HPLC column
1.50448.0001
ZIC®-HILIC (3.5µm, 200Å) 150 x 2.1 mm PEEK HPLC column
1.50449.0001
ZIC®-HILIC (3.5µm, 200Å) 150 x 4.6 mm PEEK HPLC column
1.50450.0001
ZIC®-HILIC (5µm, 200Å) 50 x 2.1 mm PEEK HPLC column
1.50451.0001
ZIC®-HILIC (5µm, 200Å) 50 x 4.6 mm PEEK HPLC column
1.50452.0001
ZIC®-HILIC (5µm, 200Å) 100 x 2.1 mm PEEK HPLC column
1.50453.0001
ZIC®-HILIC (5µm, 200Å) 100 x 4.6 mm PEEK HPLC column
1.50454.0001
ZIC®-HILIC (5µm, 200Å) 150 x 2.1 mm PEEK HPLC column
1.50455.0001
ZIC®-HILIC (5µm, 200Å) 150 x 4.6 mm PEEK HPLC column
1.50456.0001
ZIC®HILIC (5µm, 200Å) 150 x 7.5 mm PEEK HPLC column
1.50457.0001
ZIC®-HILIC (5µm, 200Å) 250 x 2.1 mm PEEK HPLC column
1.50458.0001
ZIC®-HILIC (5µm, 200Å) 250 x 4.6 mm PEEK HPLC column
1.50437.0001
ZIC®-pHILIC polymeric (5µm) 20 x 2.1 mm PEEK Guard (1 pc) PEEK保护柱(1件)
1.50438.0001
ZIC®-pHILIC polymeric (5µm) 20 x 2.1 mm PEEK Guard Kit PEEK保护柱套件(3件)
1.50462.0001
ZIC®-pHILIC polymeric (5µm) 100 x 2.1 mm PEEK metal-free HPLC column
1.50460.0001
ZIC®-pHILIC polymeric (5µm) 150 x 2.1 mm PEEK metal-free HPLC column
1.50464.0001
ZIC®-pHILIC polymeric (5µm) 100 x 4.6 mm PEEK metal-free HPLC column
1.50463.0001
ZIC®-pHILIC polymeric (5µm) 50 x 4.6 mm PEEK metal-free HPLC column
1.50459.0001
ZIC®-pHILIC polymeric (5µm) 50 x 2.1 mm PEEK metal-free HPLC column
1.50461.0001
ZIC®-pHILIC polymeric (5µm) 150 x 4.6 mm PEEK metal-free HPLC column